‘Cold SSCP’: a simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analyses

non-radioactive single-strand conformation polymorphism (sscp) analysis of relatively long pcr products

the sscp technique is based on the appearance of new “refolding” conformations during electro-phoresis due to mutation. in order to develop a simple, non-radioactive sscp analysis method so that it can reliably detect single nucleotide changes in pcr products up to 500 bp in length, extensive optimisation trials were performed . the best separation of sscp bands of pcr products up to 500 bp in ...

Carrier Determination in a Hemophilia B Family Using Single Strand Conformation Polymorphism (SSCP) and Sequencing

Single-strand Conformation Polymorphism (sscp) Analysis: a Rapid and Sensitive Method for Detection of Genetic Diversity among Virus Population

Here we present a review of the single-strand conformation polymorphism (SSCP) method, which allows DNA fragments that have been amplified with specific primers and polymerase chain reaction (PCR) to be scanned rapidly for any sequence variation. The general principles of the method are described, as well as the major factors that must be considered in developing an optimal SSCP strategy, namel...

F-SSCP: fluorescence-based polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis.

A fluorescence-based method for polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis, F-SSCP, was developed in which the target sequence is amplified by the PCR using fluorescent primers. The amplified products are then heat-denatured and applied to a water-jacket controlled gel in an automated DNA sequencer. The separated strands are detected as laser-excited f...

Carrier determination in a Hemophilia B family using single strand conformation polymorphism (SSCP) and sequencing

Hemophilia B is an X-linked recessive bleeding disorder caused by heterogeneous mutations in factor IX gene. In about one-third of cases it arises by a new mutation in germ-line cells. In this study carrier testing was performed for females of a family with only one affected individual by single strand conformation polymorphism (SSCP). Results indicated that the SSCP band shift in the propositu...